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Addgene

pSG5-Cyp26a1
(Plasmid #13457)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5-HS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cyp26a1
  • Alt name
    Cytochrome P450, family 26, subfamily a, polypeptide 1
  • Alt name
    P450RA
  • Alt name
    P450RAI
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1500
  • GenBank ID
    NM_007811
  • Entrez Gene
    Cyp26a1 (a.k.a. Cyp26, P450RA, P450RAI, RAH)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer na
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Malcolm L. Snead, UCLA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-length cDNA for mouse Cyp26a1 cloned into modified pSG5. See Author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-Cyp26a1 was a gift from T. Michael Underhill (Addgene plasmid # 13457 ; http://n2t.net/addgene:13457 ; RRID:Addgene_13457)

  • For your References section:

    BMP action in skeletogenesis involves attenuation of retinoid signaling. Hoffman LM, Garcha K, Karamboulas K, Cowan MF, Drysdale LM, Horton WA, Underhill TM. J Cell Biol. 2006 Jul 3. 174(1):101-13. 10.1083/jcb.200604150 PubMed 16818722
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