Skip to main content
Addgene

pLU-YFP-ICP0FxE
(Plasmid #134564)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134564 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLU
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP-ICP0
  • Species
    Other
  • Mutation
    RING finger deletion (FXE)
  • Tag / Fusion Protein
    • None (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Roger Everett

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pLU-YFP-ICP0FxE contains A514P and V886A differences compared to YP_009137074.1. The functional consequences of these are unknown but the protein is catalytically inert by design.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLU-YFP-ICP0FxE was a gift from Susan Janicki (Addgene plasmid # 134564 ; http://n2t.net/addgene:134564 ; RRID:Addgene_134564)

  • For your References section:

    Single-cell analysis of Daxx and ATRX-dependent transcriptional repression. Newhart A, Rafalska-Metcalf IU, Yang T, Negorev DG, Janicki SM. J Cell Sci. 2012 Nov 15;125(Pt 22):5489-501. doi: 10.1242/jcs.110148. Epub 2012 Sep 12. 10.1242/jcs.110148 PubMed 22976303
Related items:
Commonly requested with: