Cherry-ICP0FxE-C3
(Plasmid #134562)

• Purpose: Expresses Cherry-ICP0 fusion protein with a RING finger deletion (FXE)
• Depositing Lab: Susan Janicki
• Publication: Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-501. doi: 10.1242/jcs.110148. Epub 2012 Sep 12.

Backbone

• Vector backbone: pEYFP-C3; vector was modified by substituting mCherry for YFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ICP0
• Species: Other
• Mutation: RING finger deletion (FXE)
• Promoter: CMV
• Tag / Fusion Protein:
  • Cherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: blunt XhoI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: mCherry-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Roger Everett

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cherry-ICP0FxE-C3 contains a V886A difference compared to the reference sequence. The functional outcome is unknown but this enzyme is catalytically inert as designed.