YFP-Sp100A(K297A)-C1
(Plasmid #134552)

• Purpose: Expresses YFP-Sp100A fusion protein with a K297A mutation
• Depositing Lab: Susan Janicki
• Publication: Newhart et al Mol Biol Cell. 2013 Mar 13.

Backbone

• Vector backbone: pEYFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sp100A(K297A)
• Species: H. sapiens (human)
• Mutation: K297R point mutation in the SUMO site
• Promoter: CMV
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHIHI (destroyed during cloning)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • YFP-Sp100A(K297R)-C1.gb
• A portion of this plasmid was derived from a plasmid made by: Gerd Maul

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry