YFP-Rpp40-C1
(Plasmid #134549)

• Purpose: Expresses YFP-Rpp40 fusion protein
• Depositing Lab: Susan Janicki
• Publication: Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69. doi: 10.1091/mbc.E15-02-0099. Epub 2016 Feb 3.

Backbone

• Vector backbone: pEYFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rpp40
• Species: H. sapiens (human)
• Entrez Gene: RPP40 (a.k.a. RNASEP1, bA428J1.3)
• Promoter: CMV
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • YFP-Rpp40-C1.gb
• A portion of this plasmid was derived from a plasmid made by: Open Biosystems

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry