Cherry-Fibrillarin-C1
(Plasmid #134539)

• Purpose: Expresses Cherry-Fibrillarin fusion protein
• Depositing Lab: Susan Janicki
• Publication: Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69. doi: 10.1091/mbc.E15-02-0099. Epub 2016 Feb 3.

Backbone

• Vector backbone: pEYFP-C1; vector was modified by substituting mCherry for YFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fibrillarin
• Species: H. sapiens (human)
• Entrez Gene: FBL (a.k.a. FIB, FLRN, Nop1, RNU3IP1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Cherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: mCherry-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry