YFP-PML-IV-mSIM-C3
(Plasmid #134529)

• Purpose: Expresses Cherry-PML-IV fusion protein with a mutation in its SIM
• Depositing Lab: Susan Janicki
• Publication: Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs.220970. doi: 10.1242/jcs.220970.

Backbone

• Vector backbone: pEYFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PML-IV-mSIM
• Species: H. sapiens (human)
• Mutation: SIM (VVVI) 556-559 aa changed to VGGG
• Promoter: CMV
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Roger Everett

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry