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pLX311-SHOC2-V5
(Plasmid #134521)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134521 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX311
  • Backbone size w/o insert (bp) 8767
  • Total vector size (bp) 10513
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SHOC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1746
  • Mutation
    Silent mutations made at wobble base position at nucleotides 594, 600, 717, and 723 to prevent gRNA targeting
  • GenBank ID
    NM_007373.3
  • Entrez Gene
    SHOC2 (a.k.a. NSLH1, SIAA0862, SOC2, SUR8)
  • Promoter EF1alpha
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX311-SHOC2-V5 was a gift from Andrew Aguirre (Addgene plasmid # 134521 ; http://n2t.net/addgene:134521 ; RRID:Addgene_134521)

  • For your References section:

    Synthetic Lethal Interaction of SHOC2 Depletion with MEK Inhibition in RAS-Driven Cancers. Sulahian R, Kwon JJ, Walsh KH, Pailler E, Bosse TL, Thaker M, Almanza D, Dempster JM, Pan J, Piccioni F, Dumont N, Gonzalez A, Rennhack J, Nabet B, Bachman JA, Goodale A, Lee Y, Bagul M, Liao R, Navarro A, Yuan TL, Ng RWS, Raghavan S, Gray NS, Tsherniak A, Vazquez F, Root DE, Firestone AJ, Settleman J, Hahn WC, Aguirre AJ. Cell Rep. 2019 Oct 1;29(1):118-134.e8. doi: 10.1016/j.celrep.2019.08.090. 10.1016/j.celrep.2019.08.090 PubMed 31577942
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