CAP Z alpha 1 beta 1 CP
(Plasmid #13451)

• Depositing Lab: John Cooper
• Publication: Soeno et al J Muscle Res Cell Motil. 1998 Aug . 19(6):639-46.

Backbone

• Vector backbone: pET- 3d
• Backbone manufacturer: novagen
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: capping protein alpha 1 beta 1 (chicken)
• Alt name: actin CAP Z
• Alt name: CAPZB
• Species: G. gallus (chicken)
• Insert Size (bp): 2000
• Mutation: none
• Entrez Gene: CAPZB

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: nco 1 for alpha subunit. nd1 1 for beta (not destroyed)
• 3′ cloning site: bam H1 (not destroyed)
• 5′ sequencing primer: SEQUENCE
• 3′ sequencing primer: SEQUENCE

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The plasmid was a generous gift from T. Obinata, of Chiba University.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry