pet 3d alpha 1 beta 2 CP
(Plasmid
#13450)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13450 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET- 3d
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Backbone manufacturernovagen
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecapping protein alpha 1 beta 2 (mouse)
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Alt nameactin CP mouse
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SpeciesM. musculus (mouse)
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Insert Size (bp)2000
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Mutationnone
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Entrez GeneCapza1 (a.k.a. CAPZ, CAZ1, Cappa1, capZ alpha-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site nco 1 for alpha subunit. nd1 1 for beta (not destroyed)
- 3′ cloning site bam H1 (not destroyed)
- 5′ sequencing primer SEQUENCE
- 3′ sequencing primer SEQUENCE (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pet 3d alpha 1 beta 2 CP was a gift from John Cooper (Addgene plasmid # 13450 ; http://n2t.net/addgene:13450 ; RRID:Addgene_13450) -
For your References section:
How capping protein binds the barbed end of the actin filament. Wear MA, Yamashita A, Kim K, Maeda Y, Cooper JA. Curr Biol. 2003 Sep 2. 13(17):1531-7. 10.1016/S0960-9822(03)00559-1 PubMed 12956956