pCAG-CFP-SaCas9-HF (without sgRNA)
(Plasmid #134470)

• Purpose: Human expression vector for SaCas9-HF variant.
• Depositing Lab: Zongli Zheng
• Publication: Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. pii: 1906843116. doi: 10.1073/pnas.1906843116.

Backbone

• Vector backbone: pCAG-CFP
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
• Alt name: SaCas9-HF
• Mutation: R245A, N413A, N419A, R654A
• Promoter: CAG
• Tag / Fusion Protein:
  • SV40NLS-3xFLAG (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry