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Addgene

SABgeolox2DTA
(Plasmid #13447)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13447 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Mammalian Expression, Cre/Lox
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SABgeolox2DTA
  • Alt name
    lacZ-neo
  • Insert Size (bp)
    6100

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector contains a promoter trap module (SAbetageo*pA) flanked by loxP sites and PGKdtabpA. Order of elements: loxP site, adenoviral splice acceptor (SA), a lacZ-neo reporter (betageo), bovine growth hormone polyA (bpA), loxP site, PGK promoter, A subunit of diphtheria toxin gene (dta), bpA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SABgeolox2DTA was a gift from Philippe Soriano (Addgene plasmid # 13447 ; http://n2t.net/addgene:13447 ; RRID:Addgene_13447)
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