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mApple-M1 SpastinHFDD
(Plasmid #134462)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134462 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mApple-C1
  • Total vector size (bp) 6540
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    M1 Spastin
  • Species
    H. sapiens (human)
  • Mutation
    Met to Ala at amino acid 87; His to Asp at amino acid 120; Phe to Asp at amino acid 124
  • GenBank ID
    NM_014946
  • Entrez Gene
    SPAST (a.k.a. ADPSP, FSP2, SPG4)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mApple-M1 SpastinHFDD was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 134462 ; http://n2t.net/addgene:134462 ; RRID:Addgene_134462)

  • For your References section:

    Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III. Chang CL, Weigel AV, Ioannou MS, Pasolli HA, Xu CS, Peale DR, Shtengel G, Freeman M, Hess HF, Blackstone C, Lippincott-Schwartz J. J Cell Biol. 2019 Aug 5;218(8):2583-2599. doi: 10.1083/jcb.201902061. Epub 2019 Jun 21. 10.1083/jcb.201902061 PubMed 31227594
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