ROSAFARY
(Plasmid #13446)

• Depositing Lab: Philippe Soriano
• Publication: Chen et al Nat Genet. 2004 Mar . 36(3):304-12.

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418), Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ROSAFARY
• Alt name: lacZ-neo
• Alt name: betageo*
• Insert Size (bp): 6200

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (not destroyed)
• 3′ cloning site: See map (not destroyed)
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ROSAFARY=reverse orientation splice acceptor for array. This retroviral gene trap vector contains a promoter trap module (SAbetageo*pA) and a polyA trap module (PGKhygSD). Order of elements: 5' UTR, adenoviral splice acceptor (SA), a more sensitive lacZ-neo reporter (betageo*), bovine growth hormone polyA (bpA), FRT site, PGK promoter-driven hygromycin gene, adenoviral splice donor, FRT site, 3' UTR.