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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13445 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonena
- Backbone size w/o insert (bp) 2900
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Vector typeMammalian Expression, Cre/Lox
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePGKneoF2L2DTA
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Alt namePGKneobpA
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Alt namePGKdtabpA
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Insert Size (bp)3600
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector contains PGKneobpA flanked by both loxP and FRT sites and PGKdtabpA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PGKneoF2L2DTA was a gift from Philippe Soriano (Addgene plasmid # 13445 ; http://n2t.net/addgene:13445 ; RRID:Addgene_13445) -
For your References section:
Context-specific requirements for Fgfr1 signaling through Frs2 and Frs3 during mouse development. Hoch RV, Soriano P. Development. 2006 Feb . 133(4):663-73. 10.1242/dev.02242 PubMed 16421190