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Addgene

PGKneobpA
(Plasmid #13442)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13442 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGKneobpA
  • Alt name
    neo
  • Insert Size (bp)
    1700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector contains: phosphoglycerate kinase I (PGK) promoter, neomycin resistance gene (neo), bovine growth hormone polyadenylation site (bpA).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGKneobpA was a gift from Philippe Soriano (Addgene plasmid # 13442 ; http://n2t.net/addgene:13442 ; RRID:Addgene_13442)

  • For your References section:

    Promoter interactions in retrovirus vectors introduced into fibroblasts and embryonic stem cells. Soriano P, Friedrich G, Lawinger P. J Virol. 1991 May . 65(5):2314-9. PubMed 2016762
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