S3_004_026_MV1
(Plasmid #134416)

• Purpose: pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1
• Depositing Lab: Mattheos Koffas
• Publication: Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-2130. doi: 10.1021/acssynbio.9b00212. Epub 2019 Aug 28.

Backbone

• Vector backbone: pYTKMV1
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mCherry
• Insert Size (bp): 711
• Mutation: none
• Promoter: pGMP1
• Tag / Fusion Protein:
  • none

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: CTGAACTGGCCGATAATTGCAGACG
• 3′ sequencing primer: GGTTCGTAACATCTCTGTAACTGCT

Gene/Insert 2

• Gene/Insert name: FdeR-N_2xNLS
• Insert Size (bp): 948
• Mutation: none
• Promoter: pTDH3
• Tag / Fusion Protein:
  • none

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: CTGAACTGGCCGATAATTGCAGACG
• 3′ sequencing primer: GGTTCGTAACATCTCTGTAACTGCT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry