-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 13441 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonena
- Backbone size w/o insert (bp) 2900
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePGKFlpebpA
-
Alt nameFLPe
-
Insert Size (bp)2300
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This vector contains: phosphoglycerate kinase I (PGK) promoter, FLPe recombinase (Flpe), bovine growth hormone polyadenylation site (bpA).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PGKFlpebpA was a gift from Philippe Soriano (Addgene plasmid # 13441 ; http://n2t.net/addgene:13441 ; RRID:Addgene_13441)