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pBbE5a-Opto-Cre-Vvd
(Plasmid #134404)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbE5a
  • Backbone size w/o insert (bp) 3709
  • Total vector size (bp) 5720
  • Vector type
    Bacterial Expression, Cre/Lox, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MG1655
  • Growth instructions
    When transformed with Cre reporter, store in the dark.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Opto-Cre-Vvd
  • Alt name
    nCre-Vvd/Vvd-cCre
  • Insert Size (bp)
    2011
  • Promoter lacUV5

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggaattgtgagcggataacaatttc
  • 3′ sequencing primer cgttttatttgatgcctggagatcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/786533v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbE5a-Opto-Cre-Vvd was a gift from Mary Dunlop (Addgene plasmid # 134404 ; http://n2t.net/addgene:134404 ; RRID:Addgene_134404)

  • For your References section:

    Light-Inducible Recombinases for Bacterial Optogenetics. Sheets MB, Wong WW, Dunlop MJ. ACS Synth Biol. 2020 Feb 21;9(2):227-235. doi: 10.1021/acssynbio.9b00395. Epub 2020 Jan 21. 10.1021/acssynbio.9b00395 PubMed 31961670
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