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Addgene

pCS2+ MT Tcf3 (Xenopus)
(Plasmid #13439)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13439 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tcf3
  • Alt name
    HMG-box transcription factor Tcf-3
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2000
  • Entrez Gene
    tcf7l1.S (a.k.a. XELAEV_18020249mg, XTCF-3, tcf3d, tcf7l1, tcf7l1-a, tcf7l1-b, xtcf3)
  • Tag / Fusion Protein
    • Myc Tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ? (unknown if destroyed)
  • 3′ cloning site ? (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ MT Tcf3 (Xenopus) was a gift from Marc Kirschner (Addgene plasmid # 13439 ; http://n2t.net/addgene:13439 ; RRID:Addgene_13439)
  • For your References section:

    Physiological regulation of [beta]-catenin stability by Tcf3 and CK1epsilon. Lee E, Salic A, Kirschner MW. J Cell Biol. 2001 Sep 3. 154(5):983-93. 10.1083/jcb.200102074 PubMed 11524435