pAD6
(Plasmid
#134349)
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PurposeE. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134349 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepskB2
- Backbone size w/o insert (bp) 5344
- Total vector size (bp) 8792
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemfd
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SpeciesE. coli
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Insert Size (bp)3448
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Entrez Genemfd (a.k.a. b1114, ECK1100)
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 Promotor
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAD6 was a gift from Seth Darst (Addgene plasmid # 134349 ; http://n2t.net/addgene:134349 ; RRID:Addgene_134349) -
For your References section:
Crystallization and preliminary structure determination of Escherichia coli Mfd, the transcription-repair coupling factor. Deaconescu AM, Darst SA. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Dec 1;61(Pt 12):1062-4. doi: 10.1107/S1744309105035876. Epub 2005 Nov 24. 10.1107/S1744309105035876 PubMed 16511235
Map uploaded by the depositor.
