Shp2 biosensor
(Plasmid
#134346)
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PurposeFRET biosensor. To visualize Shp2 activity by FRET
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134346 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3'
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 8629
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameShp2 Biosensor
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SpeciesSynthetic
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Insert Size (bp)3229
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Shp2 biosensor was a gift from Yingxiao Wang (Addgene plasmid # 134346 ; http://n2t.net/addgene:134346 ; RRID:Addgene_134346) -
For your References section:
Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions. Sun J, Lei L, Tsai CM, Wang Y, Shi Y, Ouyang M, Lu S, Seong J, Kim TJ, Wang P, Huang M, Xu X, Nizet V, Chien S, Wang Y. Nat Commun. 2017 Sep 7;8(1):477. doi: 10.1038/s41467-017-00569-6. 10.1038/s41467-017-00569-6 PubMed 28883531
Map uploaded by the depositor.
