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Addgene

pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)
(Plasmid #134331)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134331 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG (Addgene plasmid #11179)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human codon optimized Nme2Cas9
  • Alt name
    KAC582
  • Alt name
    Nme2Cas9c
  • Species
    Synthetic
  • Promoter CAG
  • Tag / Fusion Protein
    • NLS(SV40)-3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer oBK280-CCTACAGCTCCTGGGCAACG
  • 3′ sequencing primer oBK23-CAGAGGGAAAAAGATCTCAGTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582) was a gift from Joseph Bondy-Denomy & Benjamin Kleinstiver (Addgene plasmid # 134331 ; http://n2t.net/addgene:134331 ; RRID:Addgene_134331)

  • For your References section:

    Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer. Mahendra C, Christie KA, Osuna BA, Pinilla-Redondo R, Kleinstiver BP, Bondy-Denomy J. Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26. 10.1038/s41564-020-0692-2 PubMed 32218510
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