pBSSK p85 alpha (delta SH3, iSH2)
(Plasmid
#13433)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 13433 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBluescript SK
- Backbone size w/o insert (bp) 3000
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namep85 alpha (delta SH3, iSH2)
-
Alt namep85a
-
Alt namep85 alpha
-
SpeciesH. sapiens (human)
-
MutationFlag tags replacing both the SH3 and iSH2 domains.
-
Entrez GenePIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha)
-
Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- Flag
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
see author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBSSK p85 alpha (delta SH3, iSH2) was a gift from Ronald Kahn (Addgene plasmid # 13433 ; http://n2t.net/addgene:13433 ; RRID:Addgene_13433) -
For your References section:
Phosphoinositide 3-kinase regulatory subunit p85alpha suppresses insulin action via positive regulation of PTEN. Taniguchi CM, Tran TT, Kondo T, Luo J, Ueki K, Cantley LC, Kahn CR. Proc Natl Acad Sci U S A. 2006 Aug 8. 103(32):12093-7. 10.1073/pnas.0604628103 PubMed 16880400
Map uploaded by the depositor.