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Addgene

pBSSK p85 alpha (delta iSH2)
(Plasmid #13432)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13432 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p85 alpha (delta iSH2)
  • Alt name
    p85a
  • Alt name
    p85 alpha
  • Species
    H. sapiens (human)
  • Mutation
    Dominant negative p85 alpha. The p110 alpha binding domain deleted.
  • Entrez Gene
    PIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha)
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

see author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSSK p85 alpha (delta iSH2) was a gift from Ronald Kahn (Addgene plasmid # 13432 ; http://n2t.net/addgene:13432 ; RRID:Addgene_13432)

  • For your References section:

    Phosphoinositide 3-kinase regulatory subunit p85alpha suppresses insulin action via positive regulation of PTEN. Taniguchi CM, Tran TT, Kondo T, Luo J, Ueki K, Cantley LC, Kahn CR. Proc Natl Acad Sci U S A. 2006 Aug 8. 103(32):12093-7. 10.1073/pnas.0604628103 PubMed 16880400
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