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pFudio-Cpg15-IRES2-TdTomatoW
(Plasmid #134309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFUW
  • Backbone size w/o insert (bp) 9534
  • Total vector size (bp) 11995
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    cpg15
  • Alt name
    Neuritin, Nrn1
  • Species
    M. musculus (mouse), R. norvegicus (rat)
  • Insert Size (bp)
    465
  • GenBank ID
    NM_153529.2
  • Entrez Gene
    Nrn1 (a.k.a. 0710008J23Rik, Cpg15, Nrn)
  • Promoter UbC

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Nhe1 (not destroyed)
  • 5′ sequencing primer GGACCCCGCCGCCCC
  • 3′ sequencing primer CCGCCACCATGGGACTTAAGTTGAACGG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    TdTomato
  • Species
    Synthetic
  • Insert Size (bp)
    1431
  • Promoter UbC

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asc1 (not destroyed)
  • 3′ cloning site Age1 (not destroyed)
  • 5′ sequencing primer TTACTTGTACAGCTCGTCCATGCCG
  • 3′ sequencing primer ACCGGTGCCACAACCATGGTGA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFudio-Cpg15-IRES2-TdTomatoW was a gift from Elly Nedivi (Addgene plasmid # 134309 ; http://n2t.net/addgene:134309 ; RRID:Addgene_134309)

  • For your References section:

    CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses for Stabilization by Facilitating PSD95 Recruitment. Subramanian J, Michel K, Benoit M, Nedivi E. Cell Rep. 2019 Aug 6;28(6):1584-1595.e5. doi: 10.1016/j.celrep.2019.07.012. 10.1016/j.celrep.2019.07.012 PubMed 31390571
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