pBAD-bHs
(Plasmid #134259)

• Purpose: For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag
• Depositing Lab: Edwin Manuel
• Publication: Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7163.MCT-19-0556. doi: 10.1158/1535-7163.MCT-19-0556.

Backbone

• Vector backbone: EGFP-pBAD
• Backbone manufacturer: Michael Davidson (Addgene #54762)
• Backbone size w/o insert (bp): 4698
• Total vector size (bp): 5467
• Modifications to backbone: EGFP removed by XhoI-EcoRI digest
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
• Alt name: bHs
• Species: Synthetic; Streptomyces koganeinsis
• Insert Size (bp): 759
• Mutation: codon optimized for Salmonella typhimurium
• Promoter: AraC
• Tag / Fusion Protein:
  • 6x His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TATTTATACTCGAGCATGCCGGTTGCGCGTCGTCTG
• 3′ sequencing primer: TATTTATAGAATTCTCATTACGCCGGCGCGATGGTGGTAAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry