HP138-neo
(Plasmid
#134247)
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PurposeCas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 134247 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeCRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameContains Cas9
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SpeciesOther
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HP138-neo was a gift from Iain Cheeseman (Addgene plasmid # 134247 ; http://n2t.net/addgene:134247 ; RRID:Addgene_134247) -
For your References section:
The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork of Interactions at the Centromere-Kinetochore Interface. McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM. Mol Cell. 2015 Dec 17;60(6):886-98. doi: 10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19. 10.1016/j.molcel.2015.10.027 PubMed 26698661