pKM28
(Plasmid #134226)

• Purpose: Retroviral plasmid expressing mCherry - HJURP
• Depositing Lab: Iain Cheeseman
• Publication: Cheeseman Lab Plasmids (unpublished)

Backbone

• Vector backbone: pkg183
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mcherry-HJURP-lacI
• Species: H. sapiens (human)
• Entrez Gene: HJURP (a.k.a. FAKTS, URLC9, hFLEG1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry