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Addgene

pKM499
(Plasmid #134216)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134216 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACEBac1
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    his-CENPU + CENPQ+ CENPOP
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bstx (unknown if destroyed)
  • 3′ cloning site CIP (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM499 was a gift from Iain Cheeseman (Addgene plasmid # 134216 ; http://n2t.net/addgene:134216 ; RRID:Addgene_134216)

  • For your References section:

    The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork of Interactions at the Centromere-Kinetochore Interface. McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM. Mol Cell. 2015 Dec 17;60(6):886-98. doi: 10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19. 10.1016/j.molcel.2015.10.027 PubMed 26698661
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