pDK712
(Plasmid
#134207)
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PurposeMammalian expression of EB3-tDT BMAM
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 134207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneEB3-tdTomato
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Backbone manufacturerErik Dent (Addgene plasmid # 50708)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEB3-tDT BMAM
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- tdTomato (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDK712 was a gift from Iain Cheeseman (Addgene plasmid # 134207 ; http://n2t.net/addgene:134207 ; RRID:Addgene_134207)