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Addgene

pDK521
(Plasmid #134204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACEBAC1
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Astrin 1-693-sGFP-His BroAstrin and LC8 and AltSKAP in pACE1
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDK521 was a gift from Iain Cheeseman (Addgene plasmid # 134204 ; http://n2t.net/addgene:134204 ; RRID:Addgene_134204)

  • For your References section:

    Astrin-SKAP complex reconstitution reveals its kinetochore interaction with microtubule-bound Ndc80. Kern DM, Monda JK, Su KC, Wilson-Kubalek EM, Cheeseman IM. Elife. 2017 Aug 25;6. pii: 26866. doi: 10.7554/eLife.26866. 10.7554/eLife.26866 PubMed 28841134
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