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Addgene

pDK480
(Plasmid #134197)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134197 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2eGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    untagged AltSKAP (hardened to G5siRNA)
  • Species
    H. sapiens (human)
  • Mutation
    Alternate Start, G5 siRNA hardened
  • Entrez Gene
    KNSTRN (a.k.a. C15orf23, HSD11, SKAP, TRAF4AF1)
  • Tag / Fusion Protein
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDK480 was a gift from Iain Cheeseman (Addgene plasmid # 134197 ; http://n2t.net/addgene:134197 ; RRID:Addgene_134197)

  • For your References section:

    A mitotic SKAP isoform regulates spindle positioning at astral microtubule plus ends. Kern DM, Nicholls PK, Page DC, Cheeseman IM. J Cell Biol. 2016 May 9;213(3):315-28. doi: 10.1083/jcb.201510117. Epub 2016 May 2. 10.1083/jcb.201510117 PubMed 27138257
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