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Addgene

pDK366
(Plasmid #134196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pic242
  • Backbone manufacturer
    Iain Cheeseman & Arshad Desai (Addgene plasmid # 44432)
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5xRD SKAP Alternate Start- LAP (with G5 hardeneded)
  • Species
    H. sapiens (human)
  • Mutation
    5xRD, Alternate Start, G5 hardeneded
  • Entrez Gene
    KNSTRN (a.k.a. C15orf23, HSD11, SKAP, TRAF4AF1)
  • Tag / Fusion Protein
    • LAP (GFP) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDK366 was a gift from Iain Cheeseman (Addgene plasmid # 134196 ; http://n2t.net/addgene:134196 ; RRID:Addgene_134196)

  • For your References section:

    A mitotic SKAP isoform regulates spindle positioning at astral microtubule plus ends. Kern DM, Nicholls PK, Page DC, Cheeseman IM. J Cell Biol. 2016 May 9;213(3):315-28. doi: 10.1083/jcb.201510117. Epub 2016 May 2. 10.1083/jcb.201510117 PubMed 27138257
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