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Addgene

pDK134
(Plasmid #134187)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134187 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3aTr
  • Backbone manufacturer
    Song Tan (Addgene plasmid # 64008)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KNL1 aa 69 - 479
  • Species
    C. elegans (nematode)
  • Entrez Gene
    knl-1 (a.k.a. CELE_C02F5.1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDK134 was a gift from Iain Cheeseman (Addgene plasmid # 134187 ; http://n2t.net/addgene:134187 ; RRID:Addgene_134187)

  • For your References section:

    The outer kinetochore protein KNL-1 contains a defined oligomerization domain in nematodes. Kern DM, Kim T, Rigney M, Hattersley N, Desai A, Cheeseman IM. Mol Biol Cell. 2015 Jan 15;26(2):229-37. doi: 10.1091/mbc.E14-06-1125. Epub 2014 Nov 19. 10.1091/mbc.E14-06-1125 PubMed 25411336
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