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Addgene

pKM296
(Plasmid #134180)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA5/FRT/TO
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mcherry-CENP-C 510-end-KNL2 CDK SA
  • Species
    H. sapiens (human)
  • Entrez Gene
    CENPC (a.k.a. CENP-C, CENPC1, MIF2, hcp-4)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM296 was a gift from Iain Cheeseman (Addgene plasmid # 134180 ; http://n2t.net/addgene:134180 ; RRID:Addgene_134180)

  • For your References section:

    The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork of Interactions at the Centromere-Kinetochore Interface. McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM. Mol Cell. 2015 Dec 17;60(6):886-98. doi: 10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19. 10.1016/j.molcel.2015.10.027 PubMed 26698661
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