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Addgene

pKM149
(Plasmid #134178)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134178 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3aTr
  • Backbone manufacturer
    Song Tan (Addgene plasmid # 64008)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    his-CENP-C 510-end
  • Species
    H. sapiens (human)
  • Mutation
    aa 510-end
  • Entrez Gene
    CENPC (a.k.a. CENP-C, CENPC1, MIF2, hcp-4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NDE1 (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM149 was a gift from Iain Cheeseman (Addgene plasmid # 134178 ; http://n2t.net/addgene:134178 ; RRID:Addgene_134178)

  • For your References section:

    Polo-like kinase 1 licenses CENP-A deposition at centromeres. McKinley KL, Cheeseman IM. Cell. 2014 Jul 17;158(2):397-411. doi: 10.1016/j.cell.2014.06.016. 10.1016/j.cell.2014.06.016 PubMed 25036634
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