Skip to main content
Addgene

pKM065
(Plasmid #134175)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134175 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pIC242
  • Backbone manufacturer
    Iain Cheeseman & Arshad Desai (Addgene plasmid # 44432)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-NLS-Plk1-PBD 326-603
  • Species
    H. sapiens (human)
  • Mutation
    PBD 326-603
  • Entrez Gene
    PLK1 (a.k.a. PLK, STPK13)
  • Tag / Fusion Protein
    • eGFP-TEV-S (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site SacII (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM065 was a gift from Iain Cheeseman (Addgene plasmid # 134175 ; http://n2t.net/addgene:134175 ; RRID:Addgene_134175)

  • For your References section:

    Polo-like kinase 1 licenses CENP-A deposition at centromeres. McKinley KL, Cheeseman IM. Cell. 2014 Jul 17;158(2):397-411. doi: 10.1016/j.cell.2014.06.016. 10.1016/j.cell.2014.06.016 PubMed 25036634
Related items:
Commonly requested with: