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Addgene

pKM061
(Plasmid #134174)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134174 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6p-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HJURP
  • Species
    H. sapiens (human)
  • Entrez Gene
    HJURP (a.k.a. FAKTS, URLC9, hFLEG1)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKM061 was a gift from Iain Cheeseman (Addgene plasmid # 134174 ; http://n2t.net/addgene:134174 ; RRID:Addgene_134174)

  • For your References section:

    Polo-like kinase 1 licenses CENP-A deposition at centromeres. McKinley KL, Cheeseman IM. Cell. 2014 Jul 17;158(2):397-411. doi: 10.1016/j.cell.2014.06.016. 10.1016/j.cell.2014.06.016 PubMed 25036634
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