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Addgene

pJM162
(Plasmid #134171)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134171 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJAG98 (pBABEblast)
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nde1_SSSC 284-C 318AAA320
  • Species
    H. sapiens (human)
  • Mutation
    SSSC 284-C 318AAA320
  • Entrez Gene
    NDE1 (a.k.a. HOM-TES-87, LIS4, MHAC, NDE, NUDE, NUDE1)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJM162 was a gift from Iain Cheeseman (Addgene plasmid # 134171 ; http://n2t.net/addgene:134171 ; RRID:Addgene_134171)

  • For your References section:

    Nde1 promotes diverse dynein functions through differential interactions and exhibits an isoform-specific proteasome association. Monda JK, Cheeseman IM. Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi: 10.1091/mbc.E18-07-0418. Epub 2018 Jul 19. 10.1091/mbc.E18-07-0418 PubMed 30024347
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