pJM111
(Plasmid
#134162)
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PurposeRetroviral plasmid expressing GFP - Nde1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 134162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJAG98 (pBABEblast)
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNde1_SSSC 295-C
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SpeciesH. sapiens (human)
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MutationSSSC
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Entrez GeneNDE1 (a.k.a. HOM-TES-87, LIS4, MHAC, NDE, NUDE, NUDE1)
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJM111 was a gift from Iain Cheeseman (Addgene plasmid # 134162 ; http://n2t.net/addgene:134162 ; RRID:Addgene_134162) -
For your References section:
Nde1 promotes diverse dynein functions through differential interactions and exhibits an isoform-specific proteasome association. Monda JK, Cheeseman IM. Mol Biol Cell. 2018 Sep 15;29(19):2336-2345. doi: 10.1091/mbc.E18-07-0418. Epub 2018 Jul 19. 10.1091/mbc.E18-07-0418 PubMed 30024347