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Addgene

pFbx15(-544/-527)-TK-Luc/Sox-m1
(Plasmid #13415)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13415 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTAL-Luc
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Upstream region of mouse Fbx15 gene
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    20
  • Mutation
    Mutated in Sox2 binding site

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer pGV-2U
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence of primer pGV-2U is ACT AAC ATA CGC TCT CCA TCA A.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFbx15(-544/-527)-TK-Luc/Sox-m1 was a gift from Shinya Yamanaka (Addgene plasmid # 13415 ; http://n2t.net/addgene:13415 ; RRID:Addgene_13415)

  • For your References section:

    Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Tokuzawa Y, Kaiho E, Maruyama M, Takahashi K, Mitsui K, Maeda M, Niwa H, Yamanaka S. Mol Cell Biol. 2003 Apr . 23(8):2699-708. 10.1128/MCB.23.8.2699-2708.2003 PubMed 12665572
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