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Addgene

pFR093
(Plasmid #134149)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134149 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3aTr
  • Backbone manufacturer
    Song Tan (Addgene plasmid # 64008)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CENP-T
  • Species
    H. sapiens (human)
  • Mutation
    aa 1-375 with T85D mutation
  • Entrez Gene
    CENPT (a.k.a. C16orf56, CENP-T, SSMGA)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFR093 was a gift from Iain Cheeseman (Addgene plasmid # 134149 ; http://n2t.net/addgene:134149 ; RRID:Addgene_134149)

  • For your References section:

    CENP-T provides a structural platform for outer kinetochore assembly. Nishino T, Rago F, Hori T, Tomii K, Cheeseman IM, Fukagawa T. EMBO J. 2013 Feb 6;32(3):424-36. doi: 10.1038/emboj.2012.348. Epub 2013 Jan 18. 10.1038/emboj.2012.348 PubMed 23334297
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