pFbx15(-1024/+114)-Luc
(Plasmid
#13410)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGV-BM2
- Backbone size w/o insert (bp) 4800
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUpstream region of mouse Fbx15 gene
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SpeciesM. musculus (mouse)
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Insert Size (bp)1138
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MutationNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site MunI (destroyed during cloning)
- 5′ sequencing primer pGV-2U (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sequence of primer pGV-2U is ACT AAC ATA CGC TCT CCA TCA A.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFbx15(-1024/+114)-Luc was a gift from Shinya Yamanaka (Addgene plasmid # 13410 ; http://n2t.net/addgene:13410 ; RRID:Addgene_13410) -
For your References section:
Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Tokuzawa Y, Kaiho E, Maruyama M, Takahashi K, Mitsui K, Maeda M, Niwa H, Yamanaka S. Mol Cell Biol. 2003 Apr . 23(8):2699-708. 10.1128/MCB.23.8.2699-2708.2003 PubMed 12665572
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/64/03/88b627ba-af61-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)