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Addgene

pLAI-Env
(Plasmid #133996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133996 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The bacterial strain XL10 Gold is preferred for downstream applications.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Envelope Glycoprotein
  • GenBank ID
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLAI-Env was a gift from Himanshu Garg (Addgene plasmid # 133996 ; http://n2t.net/addgene:133996 ; RRID:Addgene_133996)

  • For your References section:

    HIV-1 Env Glycoprotein Phenotype along with Immune Activation Determines CD4 T Cell Loss in HIV Patients. Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie OK, Mukherjee D, Garg H. J Immunol. 2016 Feb 15;196(4):1768-79. doi: 10.4049/jimmunol.1501588. Epub 2016 Jan 13. 10.4049/jimmunol.1501588 PubMed 26764036
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