pHP024Env
(Plasmid
#133989)
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PurposeEnv gene from HIV patient plasma in pcDNA3.1 expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133989 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe bacterial strain XL10 Gold is preferred for downstream applications.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEnvelope Glycoprotein
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GenBank IDKP754467.1
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHP024Env was a gift from Himanshu Garg (Addgene plasmid # 133989 ; http://n2t.net/addgene:133989 ; RRID:Addgene_133989) -
For your References section:
HIV-1 Env Glycoprotein Phenotype along with Immune Activation Determines CD4 T Cell Loss in HIV Patients. Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie OK, Mukherjee D, Garg H. J Immunol. 2016 Feb 15;196(4):1768-79. doi: 10.4049/jimmunol.1501588. Epub 2016 Jan 13. 10.4049/jimmunol.1501588 PubMed 26764036