pcDNA3-Flag-RNF168 delta MIU1/MIU2
(Plasmid #133981)

• Purpose: mammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deleted
• Depositing Lab: Daniel Durocher
• Publication: Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.1016/j.cell.2008.12.042.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 8484
• Total vector size (bp): 10053
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RNF168
• Species: H. sapiens (human)
• Insert Size (bp): 1569
• Mutation: deletion of MIU1 and MIU2
• Entrez Gene: RNF168 (a.k.a. RIDL, hRNF168)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • 133981
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA