Syn3D38
(Plasmid #13397)

• Depositing Lab: Thomas Weimbs
• Publication: Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Backbone

• Vector backbone: PCDNA4/TO/myc-His
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5150
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Syntaxin3D38
• Alt name: STX3
• Species: H. sapiens (human)
• Insert Size (bp): 832
• Mutation: Insert is cloned into a modified vector. First N-terminal 38 amino acids have been deleted.
• Entrez Gene: STX3 (a.k.a. DIAR12, MVID2, RDMVID, STX3A)
• Tag / Fusion Protein:
  • 2xMyc-His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMVF
• 3′ sequencing primer: pcDNA3.1/BGH reverse primier

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry