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Addgene

pSABEn
(Plasmid #133969)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133969 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYH7
  • Backbone manufacturer
    Yuhui Sun
  • Vector type
    CRISPR, Synthetic Biology
  • Selectable markers
    Apramycin for Streptomyces selection

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABEn
  • Alt name
    ABE7.10(nCas9)
  • Species
    Streptomyces

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/630137v1.full for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSABEn was a gift from Yuhui Sun (Addgene plasmid # 133969 ; http://n2t.net/addgene:133969 ; RRID:Addgene_133969)

  • For your References section:

    Base editing in Streptomyces with Cas9-deaminase fusions. Zhong Z, Guo J, Deng L, Chen L, Wang J, Li S, Xu W, Deng Z, Sun Y. bioRxiv 630137 10.1101/630137
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