pUC19-U6-terminator (SL695)
(Plasmid #133961)

• Purpose: pUC19 plasmid with U6 promoter followed by a poly-T termination signal
• Depositing Lab: Keith Joung
• Publication: Empty guide plasmid-SL695 (unpublished)

Backbone

• Vector backbone: pUC19
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U6 promoter
• Alt name: SL695
• Species: Synthetic
• Mutation: N/A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: oS280-CAGGGTTATTGTCTCATGAGCGG

Resource Information

• Supplemental Documents:
  • pUC19-U6-EMPTY (SL695).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry